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At approximately 80%% confluence (every 3 5 days depending on passage number) the cells were enzymatically harvested and re-seeded at 4,000 cells/cm.
HcoEpiC cells are very slow growing, with the cell cycle lengths ranging from 36 to 90 hours depending on passage number.
This clone expressed comparable amounts of GFP per cell compared with the p.E330K expressing cells, but was not a pure population with a varying proportion of cells not expressing GFP (depending on passage number).
Relative to Oa1+/+ and Oa1+/− cells, which appeared indistinguishable (see Materials and Methods and Supplementary Material, Fig. S1, for cell line characterization), Oa1−/− melanocytes showed the presence of macromelanosomes (Fig. 2A) and a reduced density of pigmented organelles, consistent with a lower melanin content (∼75 50% less than that of wild-type, depending on passage; not shown).
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They would also depend on passage by Congress.
The strategic plan does not depend on passage of the legislation, which has support in the Senate but has run into trouble in the Assembly, where some lawmakers are concerned about the tuition proposals.
In the coming weeks, The James Zadroga 9/11 Health and Compensation Reauthorization Act will be reintroduced to Congress and thousands of lives across the nation are depending on its passage.
The Pali canon contains three passages in which the Buddha describes pressing the tongue against the palate for the purposes of controlling hunger or the mind, depending on the passage.
Estrogen-receptor expression is altered in MCF-7 and BT474 cells depending on the passage number [ 25].
We show that protein post-translational modifications change depending on the passage number, suggesting the onset of a feedback mechanism.
The reported study shows that MADA fibroblasts accumulate different forms of prelamin A depending on the passage number.
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