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Mainly depending on experiments and tests, traditional research in materials science is costly and time-consuming.
The hydrotreating of RCR using trickle bed reactor was tested under various LHSV (0.3 1 h−1), temperature (653 693 K), and pressure (6, 8, 10 MPas) over (Ni Mo/γ-Al2O3) to determiNi Mo/γ-Al2O3on kinetocs by analyzing the results obtainedetermineng on experimentheand using kinetic models within gPreactiongram.
Depending on experiments, cells were plated on 1 µm pore size microporous PET filters (Falcon, BD Biosciences, Franklin lakes, NJ), or in plastic flasks (Falcon) or on glass lamellae (Polylabo, Strasbourg, France).
For all the purified proteins analysed, 100 μl of protein sample (1 15 nmol depending on experiments) were injected on to the column.
Thymocytes, 2 × 10 cells/ml, were incubated in RPMI 1640 medium at 37°C, 5% CO2 atmosphere with 1 µM dexamethasone for various time points depending on experiments.
Before germination, seed samples from individual plants were left at the room temperature (~24°, i.e., after-ripening) for varying lengths (0−30 d depending on experiments) to maximize the variance in a population or to estimate the after-ripening period required for a genotype to achieve 50% germination.
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The pulling movement of the machine was represented by a vertical displacement equal to 1.4 mm (depending on experiment) applied on the upper pin.
Depending on experiment, single cell suspension contained either 2.5×104 fibroblasts / ml or 5×104 SCC cells / ml.
For SEPT9 measurements on samples, reactions were performed on 10 12.5 µL of undiluted DNA depending on experiment.
However, the immunoblot signals for GnK48 and GcW1 alleles were lower (∼25 50%, depending on experiment) than for their wild-type counterparts making it necessary to normalize for input.
For all experiments outlined below, B. anynana eggs were collected from several hundred stock females and reared at either 20°C or 27°C (depending on experiment and for purely logistic reasons), high relative humidity (70±10%), and a photoperiod of L12 D12.
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