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The pulling movement of the machine was represented by a vertical displacement equal to 1.4 mm (depending on experiment) applied on the upper pin.
Depending on experiment, single cell suspension contained either 2.5×104 fibroblasts / ml or 5×104 SCC cells / ml.
For SEPT9 measurements on samples, reactions were performed on 10 12.5 µL of undiluted DNA depending on experiment.
At the end of incubation time (up to 3 hours depending on experiment), infection was stopped and non-adherent bacteria were removed by washing the cells three times with medium.
However, the immunoblot signals for GnK48 and GcW1 alleles were lower (∼25 50%, depending on experiment) than for their wild-type counterparts making it necessary to normalize for input.
The supernatant was decanted and the pellet was resuspended in Laemmli sample buffer, citrate buffer (10 mM citrate, pH 6.4) or 0.1 M Sorensen phosphate buffer, depending on experiment.
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Mainly depending on experiments and tests, traditional research in materials science is costly and time-consuming.
Depending on experiments, cells were plated on 1 µm pore size microporous PET filters (Falcon, BD Biosciences, Franklin lakes, NJ), or in plastic flasks (Falcon) or on glass lamellae (Polylabo, Strasbourg, France).
For all the purified proteins analysed, 100 μl of protein sample (1 15 nmol depending on experiments) were injected on to the column.
Thymocytes, 2 × 10 cells/ml, were incubated in RPMI 1640 medium at 37°C, 5% CO2 atmosphere with 1 µM dexamethasone for various time points depending on experiments.
The types and concentrations of plant growth regulators (PGRs) used depend on experiment (see the result section for details).
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