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A small-molecule BoNTAe inhibitor, 2,4-dichlorocinnamic hydroxamate (DCCH), was reported to have initially a Ki value of 300±12 nM [39] and most recently an IC50 value of 59 or 81 µM depending on assay conditions [40].
Assay accuracy varied considerably depending on assay and cytokine tested.
We also show that these polyP derivatives can be used to detect the action of endo- and exopolyphosphatases, depending on assay configuration.
The smallest reliably detectable cytokine differences (P < 0.05) derived from pooled interlaboratory data varied from 1.5- to 26-fold depending on assay, cytokine, and matrix type.
We now report chromogenic and fluorogenic polyphosphate substrates that permit spectrophotometric monitoring of polyphosphate hydrolysis and allow for high-throughput analyses of both endopolyphosphatase and exopolyphosphatase activities, depending on assay configuration.
This low prevalence is important because NAATs in such settings can yield a greater proportion of false positive results as opposed to true positive test results, depending on assay specificity.
Similar(49)
However, as noted above, whether or not NH4Ac is attractive depends on assay method.
This experiment was designed to investigate whether the acclimation response depends on assay conditions.
Their performance may depend on assay format and may vary across populations and settings.
However, the final steady-state rate is linear and depends on assay conditions and on the amount of added enzyme.
In vitro determination of cytostasis or cytotoxicity depends on assay conditions like doses used, incubation time and the cellular context.
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