Cell lysates were treated with or without RNase A before the immunoprecipitation to determine whether any binding interactions were dependent on association with mRNA, and the immunoprecipitated proteins were then immunoblotted with antibody to TDP-43.
Despite a similar Th2 dominance induced by alum adjuvant, it clearly was not dependent on association mucosal IgA responses.
It is also hypothesised that NF-κB is an essential 'checkpoint' of apoptosis, whose actions are dependent on association with specific transcriptional regulators.
The unexpected recovery of Pol III targets demonstrates that the roles of Nrd1 and Nab3 in surveillance are not obligatorily dependent on association with the CTD of RNA Pol II.
This mouse Mab is a broadly used pan-HLA class I-reactive monoclonal antibody that recognises a conformational epitope dependent on association between heavy chains and β2-m (Barnstable et al, 1978; Tran et al, 2001).
In parallel, we also performed TDP-1 ChIP-seq on extract pretreated with RNase to confirm the ChIP signal was dependent on association with nascent RNA and not an association with the DNA, as has been done previously (Bieberstein et al, 2014).
More recently, HER-3 phosphorylation in colorectal cancer cells was shown to be strictly dependent on association with HER-2, and that HER-2/HER-3 signalling reversed the effect of EGFR blockade on inhibiting colorectal cancer cell growth (Zhang et al, 2014).
Future research in barley root plasticity might consider the role of mycorrhizal associations in nutrient acquisition among the different domestication levels and whether wild barley plants are more dependent on mycorrhizal associations than old landraces and modern cultivars.
What is left of government funding for developing affordable homes is dependent on housing associations charging higher rents to tenants.
Activity of Cdk5 kinase is absolutely dependent on its association with one of two related regulatory subunits, p35 or p39 (Ko et al., 2001).
Furthermore, the association of the VDR genotype with IMT was not dependent on the association between VDR and BMD [ 99].
