Nevertheless, the efficacy of transient CHKA silencing in our two cellular models in inducing a dramatic drop in PCho content, as observed in si CHKA cells by H-MRS analysis, especially confirmed the dependence of EOC cholinic phenotype on elevated ChoK-alpha expression and activity.
The Kimmelman laboratory has shown that pancreatic cancers have a distinct dependence on autophagy and require elevated autonomous autophagy for tumor growth.
Repressor binding to sequences with higher in vitro nucleosome occupancy might have more elevated dependence on nucleosome dynamics and chromatin remodeling complexes [ 27, 28], and this might contribute to the better-positioned nucleosomes flanking the repressor sites in vivo.
In comparison with budded cells, Hsp104-GFP retention was significantly reduced at all temperatures when unbudded cells were exposed to elevated temperature, but the magnitude of the effect was most severe for conditions that induced curing (30°C→40°C and 37°C→40°C; Figure 5A, orange), indicating a cell-cycle stage dependence on Hsp104-GFP retention at elevated temperature.
As cancer cells develop dependence on Nampt, because of an elevated energy requirement (involving NAD-consuming enzymes), it was implied that Nampt-inhibiting molecules provide a starting point for drug discovery with Nampt as a potential target in cancer therapy.
The purpose of the current study was to determine the role of NPY in the CeA on elevated alcohol drinking produced by alcohol dependence.
We surveyed the impact of growth temperature on a panel of pex mutants and found that elevated temperature ameliorated dependence on external sucrose and reduced PEX5 levels in the pex4-1 mutant.
Add one more stage to the model: dependence on euphemisms.
Tumor growth dependence on cytoplasmic c-Fos expression was further confirmed in tumor-bearing NPcis mice which consistently show elevated expression of ER-associated c-Fos in brain cortex and in proliferating cells of PNS and CNS tumors.
These findings infer an elevated capacity to utilize other metabolic pathways in established cell lines, a lower dependence on glycolysis for energy production and thus a higher tolerance to glycolytic inhibition.
