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In order to examine the dependence of the putative classical bipartite NLS of KSHV LANA on the clusters of basic residues in Region I and II, 2×EGFP fusion constructs were prepared containing various alanine substitutions for the lysines and arginines in the background of the 51 amino acid N-terminal peptide.
Other two candidates, Cand11 and Cand12, also presented consistent evidence, but based only on HMMIR, which is heavily dependent on the folding provided by the RNAfold algorithm, and suffers from the same dependence of the putative precursor sequence as MatureBayes.
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We adopted a validated microarray protocol and applied it to a series of replicate experiments designed to test: a) the null hypothesis that the effect of any Gelsemium s. dilutions is similar to that of the control vehicle, b) whether any dose-dependence of the putative effects can be demonstrated.
To determine how the conventional methodology of lesion mapping affects the fidelity of the result we need to know the true neuroanatomical dependence of any putative function something we do not know, and cannot know, until we have precisely the model of the brain for which we need lesion mapping in the first place.
We began with the simplest possible model that can be evaluated with our data set: single voxel dependence of a putative function of interest.
The intensity dependence of the Stage II data values leads to putative null distributions which are intensity dependent and hence non-uniform.
Some of the putative culprits made biological sense — if dimly.
Molecular analysis of the putative transgenic Nicotiana benthamiana plants.
The solutions develop sharp internal layers in the vicinity of the putative interface, and are nearly constant elsewhere.
The flag of the putative Palestinian state has also been allowed to fly.
Greece's state minister Nikos Pappas hit back at criticism of the putative deal on Tuesday night.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com