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Alternatively, this difference may also be caused by higher concentration of FITC-Galacto-RGD2 (>5 μM) than that of integrin β3 antibody (2.5 μg/mL or ∼12 nM), as illustrated by the concentration dependence of staining kinetics for human cancer tissues (Supporting Information Figure SI1).
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Note that the analysis can be carried out for each stain separately so that a dependence of stain A on B but a lack of dependence of B on A can be identified and, further, that the plots permit detection of complex or mixed staining relations.
This appears to be the probable mechanism of the emission wavelength dependence of Carmine Alum staining.
As shown in Figure 1B, the dose dependence of EdU staining was found and using 100 mg/kg met the basic saturated EdU staining result.
e Photograph of stained tissue.
This concentration dependence of the SG staining that persists through boiling and SDS electrophoresis supports the formation of covalent SG albumin adducts.
Implicit flow corresponds to control and dependence of a program, i.e. stain information of variable x indirectly transfers to variable y by the conditional expression which contains x.
We have characterized linearity of the staining intensity in dependence of development time, antibody concentration, and section thickness by means of artificial standards consisting of agarose blocks into which immunogold- or alkaline phosphatase-conjugated antibodies were incorporated.
Fixation of the cells, staining of the DNA by DAPI and bright-field microscopy, as well as fluorescence imaging, revealed the shape change of the cell nucleus in dependence on the microtube diameter.
Advantages of this method against other contrast enhancing techniques and the parameters of staining are discussed.
In addition, we examined the wavelength dependence of the fluorescence intensity on a sample of a mouse intestine stained with Alexa 568 phalloidin.
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