The sample stacks were not rotated during ageing but were moved around on the shelf throughout ageing to counter any location-dependant variations in temperature and relative humidity in the chamber.
To confirm that the biological and molecular disparities observed after transmission of vCJD no. 4 reflected the propagation of two distinct prion strains, we examined the regional distribution of abnormal PrP and spongiform lesions in the brain, which are known to exhibit strain-dependant variations.
Confirmation of the characteristic GTM profiles associated with each animal, together with high degrees of microbiota stability of mature animals suggested a possible role for host-specific factors but could also be attributed to colonization-resistance of mature GTMs, resulting in the age-dependant variations in the influence of environmental change.
The advantages of using a dysplastic cell line include reproducibility, standardisation and to avoid donor-dependant variations including age and site of isolation, which often arise when using donor-derived cells.
Due to the rapid time-dependant variations of ADC, MRI can only be performed early (2 to 5 days) after the cardiac arrest, during a period when performing this examination is potentially associated with a significant risk, since patients might still require catecholamine.
However, such an age-dependant variation is not in phase with the trend of the median Hb level through the age categories: The median Hb level was quite stable from one age category to another and did not significantly decrease in 1 5-month-old infants (Fig. 3).
Better understanding of strain and location-dependant variation in murine GTMs has obvious implications for the use of animals as experimental models.
Prodon [10] gave a first example on the habitat-dependant variation in the climatic niche of birds in Europe, showing that bird species occupying holm-oak (Quercus ilex) woodlands in France had more northerly distributions (6° on average) than those occupying adjacent grassy and stony habitats.
Although the functional consequences of this preselection remain unclear, the fact that the mean CD90 expression for instance in ES medium was 11% lower in ES than in DMEM-LG (with an important donor-dependant variation as well) would, according to the ISCT minimal criteria for MSCs [ 26] imply that ES medium provides 11% less MSCs than DMEM-LG in P0.
A 4.4-fold induction of DRAK2 expression (relative to control) was observed with SC236 (5 μ M) after 4 h (P=0.001), a response that was attenuated on co-incubation with prostaglandin (SC236 vs SC236+PGE2, P=0.01), suggesting that the changes in DRAK2 expression were dependant on variations in prostaglandin generation.
