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Typically phylogenetic studies depend on sequencing one or, more usually, part of one of COI or cyt b, followed by comparison with DNA sequences held on databases (e.g. EMBL or GenBank) [32].
High-throughput community analyses do not have to depend on sequencing.
With increasing sequencing depth, error rate substantially decreases, but some errors remain and the error rates may depend on sequencing cycles, sequence context and other factors (45, 46).
While Novaes et al. caution against comparing β diversity estimates across assemblies--as they depend on sequencing depth, SNP calling criteria, and other factors--the E. propertius and P. zelicaon datasets were collected and processed in nearly identical fashion.
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We have not been able to ascertain from the literature if E was dispersed to Remote Oceania, since its identification depends on sequencing nts 16390 in HVS1.
Accuracy of assemblies, including metagenomic assemblies, depends on sequencing technology and the complexity of communities [ 65, 66].
The optimal choice of tuple size depends on sequencing depth, but it is quite robust within a range of choices for moderate sequencing depths.
De novo sequencing of transcriptomes depends on sequencing technology with long read lengths such as 454/Roche technologies to facilitate EST assembly without a genomic reference [ 27].
PCR amplification during next generation sequencing library preparation is not equally efficient across all genomic regions and depends on sequencing context.
Importantly, it depends on sequencing the pathogen, which could prove in many cases easier and more standardized than obtaining detailed clinical phenotypes.
These angles were chosen because we assume that sequence-dependent bending, bendability, and kinking would most likely depend on sequences with these rotational orientations.
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