At certain larger sample size (depending on mutant fraction and the exact size distribution of clonal expansions) RMC starts detecting individual clonal expansions, with resulting large variability between samples (this is what is apparently observed in our experiments).
In the most general case, fitness will depend on the mutant frequencies x i, as different mutants may make use of different resources, and the relative resource concentrations change as the mutant frequencies change.
The success of this approach will depend on the mutant frequency relative to the fraction of pollen grains that undergo embryogenesis; these two factors will dictate population size needed for success.
Surprisingly, we found that dimer formation did not depend on the GluN1 S108C mutant, although the presence of a cysteine residue at S108 modestly enhanced dimer formation.
However, erlotinib significantly protected PC9 cells from the toxic effects of induced mutant KRAS expression at day 7. Thus, the induction of mutant KRAS (but not induction of GFP) rescues PC9 cells from the lethal effects of erlotinib, implying that the toxicity of co-expression of mutant KRAS and EGFR depend on the kinase activity of mutant EGFR.
Predictably, the severity of inter-strand bundling disruption depends on the mutant:wild-type ratio protein concentration and on the relative position of the mutant protein incorporated into the actin filament (Fig. 6e).
Moreover, depending on the mutant gene, the risks that it will actually cause cancer can vary, from slightly above average to nearly 100percentt.
As determined by ImageJ (Abramoff et al., 2004), a small fraction of enzyme (3-12%, depending on the mutant), was not PEGylated (Figure 1, lane 5 for Cpl-1C45S D194C). Cpl-1C45S D194C
For example, depending on the mutant, the turnover number kcat was reduced by 51 68% compared with the wild-type enzyme.
