Researchers all over the world still depend on a sequencing method introduced decades ago by Fred Sanger of the Laboratory of Molecular Biology in Cambridge, U.K. It uses bacteria to amplify the DNA and expensive reagents to label bases for identification.
The future of their brainchild depended on a sequence of parachutes and rocket thrusters being deployed automatically and accurately to slow Curiosity from its atmosphere-entry speed of 13,000mph to a landing velocity of 1.7mph.
The method proposed generates without trial-and-error a family of solutions depending on a sequence of values of a parameter under consideration, e.g. reflux ratio, side-stream flows, condenser duty etc.
The TTA-UC process depends on a sequence of energy transfer processes and it is a great challenge to maintain high efficiencies while solidifying these diffusion limited energy transfer steps.
It uses finite-state transducers to create a biologically motivated similarity score which models substitutions and indels, and does not depend on a multiple sequence alignment.
+1 PRFs signals are more diverse than −1 PRFs, but still generally depend on a slippery sequence and a downstream element (e.g., secondary structure or rare codon) that causes the ribosome to pause (Dinman, 2012).
While a typical NGS analysis workflow is built on a cascade of routine tasks, individual steps are often specific for a certain assay, e.g. depend on a particular sequencing protocol.
In parallel, the entire system cannot depend on a single spreading sequence because, if it was compromised, the protection would be bypassed.
Later, successful display was shown to depend on a periplasmic signal sequence (pelB or ompA), being added to the N-terminus of the fusion protein [13].
Here we report that Ring1B belongs to the exclusive group of proteins that for their translation depend on a stable 5' UTR sequence in their mRNA known as an Internal Ribosome Entry Site (IRES).
