The deoxycytidine 5'-monophosphate (dCMP), thymidine 5'-monophosphate (dTMP), deoxyadenosine 5'-monophosphate (dAMP), and deoxyguanosine 5'-monophosphate (dGMP) standards were obtained from Sigma Aldrich (St. Lois, MO, USA).
To obtain a complex of AF0826 with a ligand, the crystals were soaked with a metabolite cocktail that contained adenine, adenosine, AMP, adenosine-5′-diphosphate ADP, ATP, 2′-deoxyadenosine-5′-monophosphate dAMP, cAMP, and ADP-ribose.
Relative to the DNA damage induced by γ rays, exposure of dA and 2′-deoxyadenosine-5′-monophosphate with γ rays resulted in significantly less amounts of α-dA relative to ODNs, indicating that the polynucleotide structure enhances the formation of α-dA.
In addition, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), and deoxythymidine triphosphate (dTTP) were individually used as substrates in 50 mM Tris HCl buffer (pH 8.5).
Inhibition of TS by 5-fluoro-2'-deoxyuridine monophosphate leads to imbalance of deoxyribonucleotide triphosphate pools (depletion of thymidine triphosphate, accumulation of deoxyuridine triphosphate and deoxyadenosine triphosphate), interference with DNA synthesis and repair, and induction of DNA strand breaks [ 2].
Another system is the cyclic adenosine monophosphate (cAMP) system.
desoxyuridylate monophosphate.
thymidylate monophosphate.
