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Optical density was then quantified with NIH Image J software.
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The RNA was then quantified and subjected to high density oligonucleotide microarray analysis using the Affymetrix HG-U133 plus 2 gene array representing ~50,000 annotated transcripts.
The relative amount of adsorbed antibody was then quantified by reaction with 2'-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and subsequent optical density reading at 405nm.
The VCDR was then quantified.
Luciferase activity as bioluminescence was then quantified.
Reduced MTT was then quantified photometrically.
The densities of hydrolysis spots are then quantified using a web-downloaded freeware (ImageJ) (Abràmoff et al. 2004).
The size, orientation and number density of the graphitic disks are then quantified using image processing techniques.
Immunoblots were then quantified by densitometry, and the density of each band normalized to a control sample treated with volunteer plasma.
The purified DNA is then quantified by checking the optical density at 260 nm and adjusted to a concentration of 150 ng/ml or higher.
Levels of CFTR were then quantified as above.
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