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The average of the TUNEL-positive cell density from two separate locations of each section (see Supplementary Figure S2) was calculated as the representative TUNEL-positive photoreceptor cell density of the section.
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In addition, RCQA indicates the density of the active section of QA-reducing PSII reaction centers.
Films were scanned using a BioRad GS-800 calibrated densitometer (Bio-Rad Laboratories, HerCAles, CA, USA), and exposure times were chosen such that the optical density range of the sections was within the pseudolinear response range of the film.
Therefore, we will revisit the density of states in the section on "Density of states for the thin quantum well" in the framework of a solvable quantum well model.
Mean-CCA-IMT was measured using an automated edge-detection algorithm based on significant changes in density of a section between the lumen and subadventitial structures perpendicular to the vessel wall.
Through monitoring the change of the occupancy status of white space channels, the RRM server can estimate the density of WSAPs, and Section 3.1 describes the extent to which the RRM server does it.
The total signal of each cell was determined by summarizing the integrated densities of the single sections of from the entire z-stack.
For each positively immunostained section, one negative control section (the next in a series of consecutive sections) was also used, and the optical density of this control section was subtracted from that of the stained section.
For each positively immunostained section, one negative control section (the following in a series of consecutive sections) was also used, and the optic density of this control section was subtracted from that of the stained section.
For each positively immunostained section, one negative control section (the following in a series of consecutive sections) was also used, and the optic density of this control section was taken away from that of the stained section.
There was no obvious difference in the density of oligodendrocytes in sections of the posterior limb of the internal capsule (A), the corpus callosum (B, C) or the cingulum (D, E) between controls (B, D) and ALS cases (A, C, E) Scale in E applies to B-D. (TIFF 4930 kb) Additional file 4: Figure S4.
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