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The PA2 cell effect (colony formation with reduced differentiation) was not observed when the cardiac progenitor cells were co-cultured with the same density of embryonic heart cells, suggesting the effect is unlikely due to the altered 3D environment.
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At an oxygen consumption of 5.6 × 10 8 mol m 2 s–1, reflecting a relatively high density of mouse embryonic stem cells, COMSOL® modeling shows that the gas permeable lid increases the uniformity of pericellular DO (Fig. 3B and 3C).
We showed that nano- and microscale moduli of RGD-functionalized alginate gels can be tailored to that of embryonic tendons by adjusting polymer concentration and crosslink density.
Density-dependent transition rates might be relevant for in vitro cultures of embryonic stem cells, which are known to differentiate when cell density becomes too large [ 8].
We show that the Src family kinase Lyn exhibits a dynamic expression pattern in the chondrogenic cell line ATDC5 and in a mixed population of embryonic mouse chondrocytes in high-density monolayer culture.
Examination of embryonic skin revealed that this lymphatic vessel density phenotype is developmentally established.
We used tandem mass spectrometry (LC-MS/MS) to quantify changes in hydroxylysyl pyridinoline (HP) and lysyl pyridinoline (LP) crosslink density in embryonic chick tendon as a function of developmental stage.
Histomorphometric analyses of embryonic and adult mutant mice demonstrate reduced long bone growth, calcium deposition, bone density, vascularity as well as delayed primary and secondary ossification.
In the glass-slide chambers with appropriate cell density, mouse embryonic cardiomyocytes formed sphere-like colonies after 3 5 days of culture (Fig. 1A).
Our initial strategy of endoderm-based recombinant growth factor-guided stimulation of embryonic stem cells extracted an intermediate population of cardiopoietic progenitors that were enriched according to the limitation of density gradients [27].
A brief treatment of embryonic development follows.
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