Trap indices represent success in a sampling effort, as the number of voles captured per 100 trap nights, i.e., a reflection of the relative population density on each sampling occasion.
Equation 4 was used to calculate the carrier density of each sample.
The thermal conductivity, effective porosity, permeability, and matrix density of each sample was determined.
It is noted here that the actual density of each sample should be higher since the calculated quantity is not considering the unobservable rods of flower-shaped structures.
Optical density of each sample was recorded at 725 nm in a UV Vis spectrophotometer (HITACHI, U-2900) using Gallic acid as standard.
Afterward, 90 μl DMEM and 10 μl CCK-8 solutions were added to each sample, and after incubation at 37 °C in humidified 5%% CO2 for 30 min, the optical density of each sample was measured at 450 nm using an auto-microplate reader (Infinite M200, Tecan, Austria).
The optical density of each sample was determined using a microplate reader set to 450 nm (Biotrack, Pharmacia).
For quantitative analysis, Oil Red O was extracted with 1 ml 99% isopropanol for 2 min, and optical density of each sample was determined at 510 nm.
Homogenates were then incubated at 50°C for 5 min, clarified by centrifugation and optical density of each sample was read at 480 nm.
The ratios of DENV-specific IgG subclass antibodies were calculated with the following formula: ratio = [(OD of sample - OD of blank) – (average of negative control)]/(OD IgG1/OD IgG2) and vice versa, where OD is the optical density of each sample.
