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Deletion of the ∆apcE gene increased the maximum cell density of cultures due to the decreased absorption of light by cells at the surface layer.
Although L. santolla showed high predation among conspecifics, cannibalism in early juvenile stages under natural conditions should be lower than in the present study, as density of cultures was high (~ 950 ind m− 2) and our experimental design provides no refuge/shelter to animals.
Cell density of cultures was determined using cell dry weight (CDW) in g/L.
Biomass Analyses, The cell concentration was monitored by measuring the optical density of cultures at 600 nm (OD600).
Visible turbidity and optical density of cultures were determined at 620 nm using NB as control to elucidate MIC [ 25].
For the Pseudomonas system, we compared the difference between optical density of cultures grown alone and cultures grown with the ancestral phage.
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The growth was evaluated by measuring the optical density of culture at 600 nm (OD600).
The amount of DNA taken up by strains varied from 0.0013 to 1.6; these numbers indicate the percent of radio-labeled DNA taken up by cells, normalized by the optical density of culture [16].
The density of culture was five embryos/mL.
by which time the optical density of culture had reached 0.5.
Bacteria were cultured in super broth containing 100 μg/mL of ampicillin at 37°C and 200 rpm until optical density of culture was 0.8.
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