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Osteogenic differentiation at day 21 and day 35 was quantified by the index of osteogenic differentiation (IOD IODOD = optical density of differentiated samples optical density of controls Chondrogenic differentiation of P3 MSCs was performed in a 3D-pellet culture system.
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The National Ocean Service in recent years has hoped to increase the density of horizontal control to the extent that no location in the United States will be farther than 50 miles (80 kilometres) from a primary point, and advances anticipated in analytic phototriangulation suggest that the envisioned density of control may soon suffice insofar as topographic mapping is concerned.
Optical density of control: absorbance of cells treated with 0.1% DMSO medium.
The cell viability was determined using the formula: {text{Viability }}left( % right) = 100 - left( {{text{optical density of sample}}/{text{optical density of control}}} right) times 100 (4) begin{gathered} {text{Optical density of sample }} = {text{ absorbance of cells treated with extract }} - {text{ absorbance of cells treated with }}0.1% {text{ DMSO medium}} end{gathered} (5).
Survival rates were calculated as the cell density in the presence of grouper β-defensin to the cell density of control.
Results were such as percent of DPPH+ scavenged, calculated by the following formula: [ optical density of control-optical density of compound)/ optical density of control)*100].
Optical density of control: absorbance of cells treated with 0.1% DMSO medium.
For heatmap analysis, a difference between optical density of control slices and treatment conditions was calculated.
The optical density of control cultures was normalized to 100% and compared with stimulated cells.
The cytotoxicity percentage was determined using the following formula: (1) % cytotoxicity = optical density of sample optical density of control × 100.
The percentage of viable cells was calculated based on the difference of the optical density of experimental group and control group with the optical density of control group.
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