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We used a stereological observer-independent procedure based on changes in volume density of cell bodies throughout the neocortex [A.
The density of cell bands was dependent on both the initial cell concentration and the pressure amplitude of the USWF.
Cell migration and transfection were simultaneously measured within hydrogels with varying density of cell adhesion sites (Arg-Gly-Asp peptides) and solids content.
In our laboratory, we recently developed a new technique to measure cell proliferation that is based on infrared spectro-photometry and the density of cell culture medium.
The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the three-dimensional (3-D) structure.
After 14 days of ODM culture, a significant increase in cell number and a higher density of cell distribution in the scaffold were observed after both static and dynamic cultivation compared to GM culture.
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This eECM allows us to independently tune the density of cell-adhesive RGD ligands presented to Caco-2 cells as well as the mechanical stiffness of the eECM.
We titrated the density of cell-mimics in a fixed volume and found a sharp transition from off to on, which resembled bacterial quorum sensing responses to cell density31 even though, unlike in bacterial quorum sensing mechanisms, the T3 activation circuit did not contain positive feedback.
These results suggest that COOH- and NH2-SAMs allow for both cell adhesion and cell spreading because of the high density of cell-binding domains derived from adsorbed Vn.
The results were shown as a percentage cell growth calculated as follows: % cell growth = (optical density of an ACNU-treated or TMZ-treated well−optimal density of cell-free control)/ optical density of cell-only control−optimal density of cell-free control)×100.
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