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The density of all the three groups of nail samples was measured with a density kit (MS DNY 54, Mettler Toledo, Greifensee, Switzerland), integrated with an electronic balance (MS105DU, Mettler Toledo, Greifensee, Switzerland).
Surface porosity was calculated from density values which were measured with a density kit and an electronic balance, while total porosity was quantified using micro-computed tomography.
diameter disks, and their volume, corresponding to the relaxed state (Vr), was determined using a heptane density kit (Vogayer Pro model VP114CN OHAUS Corporation, Pine Brook, NJ, USA).
The density of 1.35g cm−3 was determined with a density kit mounted on an analytical balance (Mettler-Toledo, Greifensee, Switzerland).
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The burial density, tool kit, ceramics, and midden fauna suggest a largely sedentary population with a subsistence economy based on fishing and on hunting of a range of savanna vertebrates.
The data were normalised to net integrated pixel density of kit-supplied internal positive controls.
The gradients were calibrated using colored beads with densities of 1.018, 1.033, 1.049 and 1.062 g/ml (Percoll Density Marker Bead Kit; Sigma-Aldrich).
PBL were harvested using a density gradient centrifugation kit (NycoPrep 1.077A, Axis-Shield, Oslo, Norway), washed several times and resuspended in PBS.
The CD34+ cells from both sources were isolated using a MACS isolation kit (density, 1.077; Pharmacia Biotech, Uppsala, Sweden) using an antibody against CD34 according to the manufacturer's instructions.
Median density of c-Kit-IR ICC was 0.00 versus 2.58 (z = −2.98, p = 0.004) in the circular muscle layers and 0.00 versus 1.84 (z = −2.98, p = 0.004) in the longitudinal muscle layers, for patients and controls, respectively.
For calculating HPC markers (e.g. EpCAM, OV6, CD133 and c-kit) density, the cutoff for the definition of subgroups was the median IOD to separate high HPC markers' expression (IOD > median) from low HPC markers' expression (IOD ≤ median) in microarray tissue samples.
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