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Band expressions were developed using Pierce ECL reagents (Thermo Scientific, Rockford, IL) and the band densities were quantitated using ImageJ analysis (National Institute of Health, Bethesda, MD).
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Band density was quantitated using the Quantity One software (Bio-Rad Laboratories, HerCAles, CA).
Protein concentration was quantitated using the BCA Protein Assay Kit (no. 23225, Thermo Fisher Scientific).
Bands were quantitated using the integrated density function of ImageJ.
Proteins were quantitated using the Infinite m200 (Tecan).
Serum levels of PEG-HCCs were quantitated using a PEGylated protein ELISA kit (Enzo Life Sciences).
Blots were quantitated using ImageJ software (NIH) or iBright Analysis software.
Human leukocyte I and i antigens were quantitated using 125I labelled purified antibodies.
Arrays were quantitated using GenePix (http://www.moleculardevices.com).
Protein concentrations were quantitated using the Bradford assay (BioRad).
Proteins were quantitated using the 2D Quant Kit (GE Healthcare).
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