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The densities of bands were analyzed with NIH Image J software and normalized to internal control.
The gene expression levels were determined by comparing densities of bands after agarose gel electrophoresis of the PCR products.
Equal loading of samples was confirmed by staining gels for protein (GelCode Blue, Pierce) and comparing the optical densities of bands using TotalLab (Non Linear Dynamics, Newcastle, UK).
Densities of bands were measured with NIH ImageJ (http://rsb.info.nih.gov/ij).
All immunoblots were scanned and optical densities of bands were measured.
Densities of bands of interest were expressed relative to the intensity of the loading control (tubulin).
Similar(42)
Density of bands was quantified by AlphaDigiDoc software.
The density of bands was measured using Image J 1.37v (Wayne Rasband, NIH) software.
The density of bands was quantified using a Multi Gauge software (Fuji Film Global).
The density of bands was quantified with Multi Gauge software (Fuji Film Global).
The optical density of bands was measured using imageJ software and the associated graphs were presented (Figures 4B 4D).
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