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Restriction site associated DNA sequencing (RADseq) was used to genotype 263 (cross 1) and 261 (cross 2) progeny at ∼8500 single nucleotide polymorphisms (SNP) and construct two dense linkage maps.
These studies are enabled by the genome-wide molecular characterization of the germplasm (Lee et al. 2004; Yi et al. 2006; Kim et al. 2008) and require enough molecular markers for the production of dense linkage and association maps to identify these QTLs.
The map is anchored to a dense linkage map consisting of 994 markers.
The platform can effectively generate dense linkage maps for QTL analysis [ 49- 51].
Here, we present a dense linkage map for Astyanax mexicanus generated using genotyping-by-sequencing technology.
To our knowledge, this map is one of the three most dense linkage maps in cotton.
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Electron dense linkages between both membranes can be seen at various locations throughout the tomogram.
Distribution of length measurements of OMM-PVM intermembrane space and electron dense linkages.
In Populus, the most gene-dense linkage maps reported had 324 genes mapped by SFPs [ 24] or 307 unique genes mapped by SNPs [ 37].
As a last observation, despite the marker-dense linkage map and the threshold values considered for QTL detection, the presence of spurious QTL cannot be completely discarded due to the limited number of individuals sampled.
New QTL analyses for group II and X with the denser linkage maps were conducted.
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