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Positive dengue samples were then subjected to plaque reduction neutralization test (PRNT) [ 15].
Regarding cross-reactivity, the best results using serum samples were found among reactive dengue samples, most likely due to the absence of HBsAg-reactive samples and/or the low number of dengue samples under analysis (i.e., due to beta error).
The NS1 sensitivity varied among confirmed dengue samples collected in different cities, ranged from 4.2% to 88.9% (Table 2).
Most of the dengue samples tested at Genetech were received from private hospitals and clinics in Colombo.
We observed a relatively low sensitivity of NS1 ELISA for dengue detection on RT-PCR-positive dengue samples.
Solid phase ELISA using the recombinantly expressed antigen with positive and negative dengue samples showed that the expressed protein retains its antigenic and immunological properties.
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Consequently, we designed and fabricated a microfluidic chamber array structure and were able to compartmentalize the bulk aqueous dengue sample into femtoliter volumes using such a device.
In the 3-dimensional MDS plot the non-dengue samples (green spheres) are grouped close together, with only one sample outlier.
The specificity of NS1 assay for non-dengue samples were 100%.
Welch's two-sample t-tests were used for comparisons among the diagnostic groups: Non-Dengue vs. Dengue (DF+DHF) samples; DF vs. DHF samples and so on (Supplement material S4) but we will focus the discussion on the comparison between DHF versus DF.
The sera purchased from Sera Care Life Sciences (Milford, MA, USA) to prepare the rest of dengue positive samples in the panel (samples #2, #7, #9, #4. #12, #13, and#3) were obtained from volunteers at FDA-regulated donation centers, with strict adherence to federal HIPAA Health Insurance Portabilityy and Accountability Act) regulations.
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