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Consequently, we designed and fabricated a microfluidic chamber array structure and were able to compartmentalize the bulk aqueous dengue sample into femtoliter volumes using such a device.
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Positive dengue samples were then subjected to plaque reduction neutralization test (PRNT) [ 15].
We observed a relatively low sensitivity of NS1 ELISA for dengue detection on RT-PCR-positive dengue samples.
The NS1 sensitivity varied among confirmed dengue samples collected in different cities, ranged from 4.2% to 88.9% (Table 2).
Most of the dengue samples tested at Genetech were received from private hospitals and clinics in Colombo.
In Singapore, dengue infections were predominantly due to dengue serotype 1 (detected in 75%to100%0% of dengue samples collected each month) during the epidemics in the year 2004 to 2006, and dengue serotype 2 (detected in up to 91% of dengue samples) during the epidemic in the year 2007 and 2008 [ 11].
Solid phase ELISA using the recombinantly expressed antigen with positive and negative dengue samples showed that the expressed protein retains its antigenic and immunological properties.
Dengue samples were tested for the presence of the viral NS1 protein using the Platelia™ ELISA (BioRad, Marnes-la-Coquette, France) following the manufacturer's instructions.
The RT-LAMP assay detected DENV genome in 74 of 171 (43.3%) of the acute dengue samples compared to 80 of 171 (46.8%) by qRT-PCR assay (Table 2).
In order to avoid discrepancies, only primary dengue samples that were within the first 7 days after the onset of illness were tested.
During the study period from 2005 to 2008, Singapore experienced two predominant serotypes at different period; serotype 1 was detected in 75 100% of dengue samples during the epidemics in the year 2005 2006 and dengue serotype 2 was detected in up to 91% dengue samples during the epidemic in the year 2007 and 2008.
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