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We observed a relatively low sensitivity of NS1 ELISA for dengue detection on RT-PCR-positive dengue samples.
The increased sensitivity of dengue detection using combination of NS1 and IgM has been previously reported [ 23- 25] and our result was in accordance with those reports.
This study described the performance of Panbio Dengue Early NS1 and IgM Capture ELISA assays for dengue detection during our surveillance in eight cities in Indonesia as well as the genetic diversity of DENV NS1 genes and its relationship with the NS1 detection.
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The results demonstrated that the SiO2@APTES-GO electrode material led to enhanced dengue RNA detection sensitivity with selectivity and detection limit (1 femto-Molar), compared to both APTES-GO and APTES-SiO2.
This is a first national operational research project with objectives to study the feasibility of implementing modern dengue outbreak detection methodologies and to evaluate these methods at better alternatives to the current outbreak detection method (median-5-years) in Thailand.
The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection.
Spin-coated chips were used for antibody (Ab) immobilization in order to investigate the influence of COOH surface concentration on dengue virus detection efficiency in enzyme-linked immunosorbent assay (ELISA) experiment.
Evaluation of Possible Dengue Outbreak Detection Methodologies for Thailand, which one should be implemented?
Of 440 samples tested, 106 were positive for NS1 antigen and 188 samples were positive by dengue genome detection and/or by virus isolation.
Interpretative criteria are given in the work of Mayxay and others. 1 Dengue genome detection and serotyping were performed using TaqMan reverse transcription polymerase chain reaction (RT-PCR) systems as described previously.
With new, sensitive and specific RDTs for dengue NS1 detection, 1, 10 it is likely that they and fluid management training would be the key clinical interventions for such rural hospitals.
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