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Available evidence does not support endemic presence of the virus in an urban dengue cycle.
The rainfall and minimum temperature spectra in Figure 4C,D demonstrated a strong annual signal (1-year periodic band), in agreement with the annual dengue cycle in the region.
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This finding was expected, as previous studies have documented significant annual dengue cycles in this region [ 26].
The 1-year cycles of minimum temperature and rainfall likely contributed to the annual dengue cycles observed in the power spectrum.
Within forest habitats of West Africa, Malaysia, and probably Vietnam, zoonotic, sylvatic dengue cycles have been described involving Aedes spp. mosquitoes and monkeys (8 – 10 ).
However, we cannot completely rule out the possibility that low levels of relocalized cytoplasmic RHA, below detection levels by immunofluorescence, may have affected the dengue life cycle.
No effective vaccines or antiviral drugs are currently available, impeding a direct intervention on the human link in the dengue transmission cycle.
The timing of dengue epidemic cycles was less spatially dependent.
Climatic factors play a significant role in the mosquito biology, the viruses they transmit, and more broadly, dengue transmission cycles.
This study focuses on the relationship between local weather dynamics and critical change-points in seasonal dengue transmission cycles.
Here, we review the recently acquired knowledge on the molecular and cell biology of the dengue virus life cycle based on newly developed molecular biology technologies.
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