A more rigorous demonstration of this requirement is still an open matter, and indeed rigorous proofs that particular solution types can only occur when three (or more) timescales are present have not, to our knowledge, been provided in the literature to date.
This is the first demonstration of a requirement for a second flagellum system for growth at low temperature.
To our knowledge, this is the first demonstration of a requirement for a specific extracellular matrix heparan sulfate proteoglycan in triglyceride metabolism in vivo.
In summary, this work provides the first clear demonstration of a requirement for non covalent SUMO-interaction motifs for SUMO E3 activity.
This is the first demonstration of the requirement for the p32 protein in the maintenance of the morphology of both the mitochondrial and ER networks.
The clear demonstration of the requirement of ErbB3 as a partner for ErbB2 in breast cell transformation and the observation that the proliferative drive requires signalling through the PI-3K pathway are in line with previous findings.
Given the emerging roles for extracellular traps in a range of disease states and the universal role of macrophages in promoting inflammation, further demonstration of the requirement for PAD2-mediated histone deimination in MET production suggests that PAD2 inhibitors may prove to be ideal therapeutics for a range of inflammatory diseases.
Thus, in spite of extensive documentation of constitutive knockout phenotypes [ 9, 17, 18] and our own conditional studies bypassing requirements prior to NOCD [ 4], formal in vivo demonstration of a direct requirement for Met in axon growth and path-finding is still currently lacking.
Generation of the Cbfb f allele and demonstration of a postnatal requirement for both CBFβ and Runx1 in C-LTMR development.
Although it has been shown that motor axons are required for the expression of arterial and smooth muscle markers (Mukouyama et al., 2005), to the best of our knowledge this is the first demonstration of a direct requirement for axons in vascular guidance and lymphangiogenesis.
The improved image clarity and tissue differentiation in a number of situations has dramatically increased the range of diagnostic information and in many cases the demonstration of pathology without the requirement of invasive tissue sampling (histology).
