The definitive demonstration of multipotency for a cell is the ability to differentiate towards more than one cell type.
Following our in vitro demonstration that CXCL12 mRNA in endothelial cells was reduced by short term exposure to DP, we tested the effect of DP on microvascular endothelium in vivo by use of laser-capture microdissection (LCM).
The scientists at the University of Florida said their experiment was a first demonstration that the cells were as valuable as researchers had said they would be in treating disease.
In the germinal cradle of the ovary, some of the meiotic maturing oocytes undergo apoptosis in sox9b-expressing somatic cells, which was similar to our demonstration that apoptotic cells were phagocytosed in male Sertoli cells.
For example, the value of CD25 as a Treg marker, particularly in tumor and autoimmune diseases, including SLE, has been questioned after the demonstration that CD25high cells are contaminated by activated effector cells [ 8, 34, 35].
This phenomenon was first described in human acute myeloid leukemia cells, by isolation of rare leukemia initiating cells that have differentiation and proliferative capabilities similar to leukemic stem cells, and demonstration that these cells are responsible for the regenerative, self-perpetuating, and diverse nature of the tumor.
Wide interest was generated in the influence of mechanical cues on cell differentiation following the demonstration that stem cells are primed for differentiation by the stiffness of their substrate, with rigid, stiff, or soft substrates supporting osteogenic, myogenic, or neurogenic differentiation, respectively.
Additionally, the use of rInsII-cre: ROSA-loxP-stop-loxP-GFP mice led to the novel demonstration that once cells were marked by insulin II promoter expression, they did not deviate from the path of becoming beta cells, even though some of them expressed multiple other islet hormone genes.
However, demonstration that these cells are functional PGCs via ablation experiments is complicated by the fact that they arise at the inner face of the blastoderm at the gastrulation center, so that their physical disruption would likely also compromise mesoderm formation and subsequent embryogenesis.
The recent demonstration that tumor cells are able to induce pericyte maturation of the neovasculature during early formation of micrometastatic foci [ 24] might provide one explanation for the lack of efficacy of bevacizumab in eradicating occult metastatic disease in colorectal cancer.
