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CRH/DH44 have been implicated as being related to mollusc ELH [ 10], demonstrating sequence similarity within the mature peptides.
The nucleotide sequences were analyzed by using ClustalW (www.ebi.ac.uk/Tools/clustalw2) to produce a neighbor-joining tree demonstrating sequence relationships.
Yet, overall, the members of the PhVC are best characterized as those demonstrating sequence variability between subtypes with no clear functional relation between them.
In Region I potential ORFs BA-0356/7/8 and BA_0361/2, demonstrating sequence homology to genes encoding an epimerase and a dehydrogenase, respectively, were identified by SSH clones 190/N, Y4 and 142/I6.
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This association is fully competed by addition of an excess of unlabeled camCaRE or cCaRE but not by addition of excess unlabeled mutant mCaRE, demonstrating sequence-specific binding of CaRF to the camCaRE probe.
An example is given in Figure 3, demonstrating sequenced isomiRs for miR-1, which indicated that the majority of porcine miRNA nucleotide variants resulted from post-transcriptional modifications.
(F ) Gel shift assay demonstrating sequence-specific binding of Zfp335 protein to labeled Z1 probe.
A sequential drug treatment strategy was chosen based on previous studies demonstrating sequence-specific synergistic effects with administration of combination of chemotherapy (doxorubicin) and Cdk inhibitor P276-00 (data not shown).
Sequence analysis using various cultivated and wild rice of the sd1 gene, which contributed to the "Green Revolution" in rice varieties and their wild progenitors, has also demonstrated sequence diversity, which is correlated with taxonomic classification.
Phylogenetic analysis demonstrated sequence homology with previous LBV isolates from South African bats.
Additional file 4: Figure S13 Alignment of all unique sequences from AhLOX7 3' amplification (2186/2187) demonstrates sequence variation.
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