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(a) Immunofluorescence staining of AFDC and MDC, demonstrating expression of the mammary cell markers vimentin (expressed by both populations), αSMA, CK14 (expressed only in AFDC), ERα and CK18 (expressed only in MDC).
Differentiated RGC-5s also had prominent TrkB staining, demonstrating expression of the high-affinity BDNF receptor.
This is a picture of primate dorsal root ganglia neurons demonstrating expression of the opiate analgesic peptide leu-enkephalin after application of a recombinant herpes virus encoding the gene for human preproenkephalin to the skin.
These results are consistent with other published reports demonstrating expression of GATA4 and GATA6 in various smooth muscle phenotypes [39], [61].
The generation of the murine vLINCL cell panel is described in detail in Materials and Methods, along with the characterization demonstrating expression of the neural stem cell marker, nestin, confirming a neuronal lineage (Figure S1).
β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and α-tubulin have been traditionally used as control, 'house-keeping' genes for Northern blotting and other conventional, less sensitive assays, in spite of decades of reports clearly demonstrating expression profiles that vary markedly based on cellular phenotype and experimental design [10] [12].
Similar(38)
First we demonstrate expression of ChR2 in either PV-expressing interneurons or callosally-projecting pyramidal neurons.
Northern blot analysis demonstrated expression of the variant mRNAs.
Use improvisation and role play: Get students to read a text aloud in different ways to demonstrate expression and intonation.
We next hypothesized that C. albicans may also demonstrate expression heterogeneity and bimodality that is linked with expression in the corresponding macrophage cell.
Transcriptome analyses showed that 11,328 of the oligonucleotides demonstrated expression in at least one tissue.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com