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More importantly, secretion of a mineralized matrix, as shown by positive alizarin red S staining, was observed demonstrating differentiation of MSC towards osteoblasts.(Figure 1C).
WtES cells exhibited expression of Ttr, Fgf5, Zg, T and Afp and lack of Oct3/4 transcripts, demonstrating differentiation of a significant proportion of cells within the population (Figure 1C i)).
When inoculated in mice, xenografts from TICs after 4 weeks contained between 0.2% and 0.5% PKH-26 labeled cells (Figure S4D, Materials and Methods S1), demonstrating differentiation of most TICs but also self-renewal of a small subset of TICs.
High-density pellet cultures confirmed these results, demonstrating differentiation towards the hyaline cartilage lineage for both ND and OA chondrocytes.
16 The one female patient where donor cells could be identified by FISH showed populations of transplanted cells that were labeled with SOX2, suggesting maintenance of the stem cell properties, and NeuN demonstrating differentiation into neuronal lineage after transplantation, which is consistent with the data from animal models.
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The negligible effect of HNSCC treatment on the activity of the above-mentioned genes, based on the level of cRNA, can be interpreted according to our recent results demonstrating differentiation-dependent expression of the endogenous lectin galectin-9 in normal squamous cell epithelium and in cancer [ 58].
We demonstrate differentiation by stiffness of individual elastic lamellae and vascular smooth muscle.
Gene expression analysis for chondrocytic-cell marker genes demonstrated differentiation of MSCs to a phenotype which showed similarities to both articular chondrocytes and NP cells.
At higher dose, this combination failed to demonstrate differentiation of safety and efficacy at ED max which may limits the usage and potential.
In this paper, we have demonstrated differentiation of hiPSC into a spectrum of region-specific neural progenitors, which further develop into functional neurons.
In this study, we demonstrated differentiation of MSCs to an NP-like phenotype and formation of appropriate NP ECM components.
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