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Finally, NIH 3T3 fibroblasts were seeded on the obtained nanofibers, demonstrating cell biocompatibility and proliferation.
Namely demonstrating cell survival, safe proliferation, and differentiation under long-term physiological conditions, where complex constructs are subject to loading forces experienced by native cartilage.
Demonstrating cell repulsive effect at the mPEGA compositions higher than 7%, the modified surfaces improved MC3T3 cell attachment, proliferation and differentiation, which reached maxima at the mPEGA composition of 5 7%.
Because the most direct method of demonstrating cell fusion is to ascertain mixing of cellular constituents of the interacting partners, these assays have in common the measurement of a new signal output only after such mixing occurs.
Consistent with EM data demonstrating cell wall-like outgrowth containing cellulose (Figure 4B and C), cellulose synthases, cellulose synthases-like, and extensins were strongly up-regulated (Figure 5C D).
However, co-expression with altNF2d9 resulted in up to a 3.5 fold increase in transactivation over that of altNF2d9 alone, not the suppression of activity that was observed in ES and HEK293T cells, demonstrating cell type-specific activity.
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Progenitors from the anterior cortex were smaller, less motile, and produced smaller clones compared to those from the posterior cortex, demonstrating cell-intrinsic differences that may contribute to the areal organization of the cerebral cortex.
First, DNA incorporation of nucleoside analogs or nuclear expression of cell-cycle proteins, while demonstrating cell-cycle activity, does not necessarily translate into genuine cell division and proliferation.
Moreover, we demonstrated cell sorting by the combination of gel-valves shown in Figure 10(b).
The osteoblast cell adhesion test demonstrated cell spreading which is considered favourable for cell proliferation and differentiation.
Furthermore, we demonstrated cell sorting by using two gel-valves fabricated near the branch of a microchannel on a chip.
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