Sentence examples for demonstrating a requirement from inspiring English sources

First, transients were reversibly abolished by chelating extracellular Ca2+, demonstrating a requirement for Ca2+ influx across the plasma membrane.

Depletion of Themis2 protein by RNA inteference inhibited LPS-induced TNF production in primary human macrophages demonstrating a requirement for Themis2 in this event.

In addition, expression of the dominant negative mutant of Ras (Ras S17N) inhibited influenza virus infection, whereas expression of its wild-type counterpart rather enhanced the infection (Figure 5D, E), together demonstrating a requirement for Ras in influenza virus infection.

Wound healing was impaired in NAS mice and in normal mice treated with a γ-secretase inhibitor, and was enhanced in mice treated with the Notch ligand Jagged, demonstrating a requirement for Notch signaling for normal wound healing.

Kinase activity was readily detected in the presence of MgCl2 and MnCl2, but not in the presence of CaCl2, ZnSO4 or EDTA (Fig. 2C), demonstrating a requirement for Mg2+ and Mn2+ cations.

Tregs from GITR−/− mice did not show a significant block in trafficking when transferred into DTA-1 treated animals, compared to IgG controls, demonstrating a requirement for direct GITR ligation on Tregs themselves (Figure 4B).

In keeping with its expression patterns, tmem33 knockdown demonstrated a requirement for tmem33 during normal angiogenesis and pronephric development.

Likewise, a decoy ODN designed to sequester activated CREB protein definitively demonstrated a requirement for CRE-mediated transcription in light signaling.

In a previous study we demonstrated a requirement for activation of mTORC1 in the stimulation of eIF2Bɛ mRNA translation in skeletal muscle in response to resistance exercise.

The results demonstrate a requirement for LPS in building arbor complexity and suggest a key role for pre-synaptic LPS in assembling neural circuits.

These results demonstrate a requirement not only to explore second-order CMC modeling approaches for such flames, but also to examine the application of different kinetic schemes, including a detailed investigation of the mechanisms and rates employed for NO chemistry.