More importantly, these modifications do not alter the level of transgene expression in non-NA cells either in vitro or in vivo, demonstrating tight cell-type specificity.
We demonstrated tight coupling between the modular chassis and ester modules for enhanced product biosynthesis, an engineered phenotype useful for directed metabolic pathway evolution.
A fluidic MEMS module – a prototype gas reformer – was bonded onto the carrier to demonstrate tight gas connections at elevated temperature.
Xylitol production by the wild-type strain (W3110) expressing CbXR is not reduced when xylE and xylG are deleted, demonstrating tight regulation of the xylose transporters by CRP and revealing significant secondary xylose transport.
ECCs demonstrate tight crack width and development of these cracks in fact increase ability of ECCs to resist effects of hot, frost and humid weather conditions besides its low permeability coefficient and higher resistance to steel corrosion compared to other common substitutes.
We confirmed the expression of the K13-ERTAM protein by immunoblotting (Figure 1A) and demonstrated tight regulation of its activity upon 4OHT treatment by immunofluorescence staining for p65/RelA subunit of NF-κB.
However, based on the DNA binding data for both condensin and the condensin-like bacterial mukB complexes which demonstrate tight DNA-SMC head interactions, as well as imaging of the condensin complex to DNA which reveals the association of the head domain to DNA, it is possible that the Smc1 and Smc3 heads bind to chromatin [20], [32], [33].
In particular, large necrotic volumes in ductal carcinoma in situ (DCIS) demonstrate tight correlation with comedo as opposed to non-comedo DCIS [ 8].
In both the repressor and activator configurations, we have demonstrated tight control of gene expression both in transient and stable expression.
Upon removal of ethylene, transcript and protein levels for ARGOS-GFP and ARL-GFP rapidly drop, demonstrating tight control of expression by ethylene (Fig. 2b).
