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Interestingly, VEGF121/rGel demonstrates targeted toxicity in vitro to endothelial cells which over-express VEGFR-2 (IC50 = 0.5 - 1 nM) but not to cells which over-express VEGFR-1 (IC50 = 300 nM) compared to gelonin alone (IC50 = 300 nM) [ 10].
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Efforts in engineering bacteriophages and synthetic probiotics demonstrate targeted antimicrobial approaches that can be fine-tuned using synthetic biology-derived principles.
Using MECA79 conjugation, we have demonstrated targeted delivery of tacrolimus to the lymph nodes following systemic administration, with the capacity for immune modulation in vivo.
Cytosolic thioredoxin reductase activity measurements were performed to demonstrate targeted inactivation of Txnrd1 in neuronal cells (Fig. 6 C).
Here, we demonstrate targeted detection of colonic dysplasia in vivo using a novel fluorescence-labeled peptide selected with phage display methodologies.
Here, we take the next step: demonstrating targeted delivery into tumor cells in vivo.
Here, we demonstrate targeted mutagenesis of endogenous Arabidopsis genes using TALENs.
VEGF121/rGel demonstrated targeted toxicity even after a one-hour exposure, with an IC50 of 5 nM.
We demonstrated targeted insertion and inversion methods in the BGM system to add to its repertoire of genetic modification approaches.
We successfully demonstrated targeted deletions, inversions, and duplications of multiple gene clusters as well as large chromosomal deletions exceeding 9 Mb.
Our findings demonstrate targeted and programmable gene repair in the human genome by delivery of both 'scissors' and 'patch' in a single combined protein and gene vehicle.
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