The first demonstration of wide-field FLIM to read microarrays was demonstrated using time-gated imaging to improve the sensitivity for DNA profiling.[ 24] More recently, an automated instrument for unsupervised FLIM of multiwell-plate sample arrays was reported[ 25] that exploited the frequency domain (FD) fluorescence lifetime determination of FRET in a wide-field (non-sectioned) microscope.
These authors, furthermore, demonstrated, using time-lapse cell-tracking microscopy, that by looking closer into the NC fraction, there are different cell types present, i.e., vacuolated, giant and polygonal cells that differ in cell proliferation and speed of cell motility.
The dynamic mesh adaptation capabilities of the approach are demonstrated using adaptive time-dependent simulations of the Orszag–Tang vortex problem with high-order accuracy and unprecedented effective resolution.
Map1b differential regulation at 24 hours from injury, as demonstrated using real-time RT-PCR and Bead-array analysis, results from its up-regulation in G93A-SOD1 spinal cord and from its down-regulation in WT spinal cord.
More recently, autonomous circadian rhythms in mouse articular cartilage tissues (and human chondrocyte-derived cell lines) have been unequivocally demonstrated using real-time bioluminescence photon counting of explant cultures of xiphoid and femoral head cartilage [ 9].
In particular, the upregulation of AAMP, angio-associated, migratory cell protein gene, in high grade DCIS with necrosis was demonstrated using array technology, real-time PCR, and in situ hybridization – a gene considered to function in migrating cells and which may be hypoxia-mediated in tumors.
The use of different metrics, including modified versions of some common landscape metrics such as contagion, that describe the distribution of blobs in space and time, is demonstrated using both model and empirical data.
The performance of the proposed ST-based measures is demonstrated using a pair of synthetic time series.
The tracking performance was demonstrated using a computer simulation of varying time delays in a bilateral teleoperation system.
The efficacy of the proposed method is demonstrated using synthetic as well as Real Time Digital Simulator test data.
