Two examples come from species (e.g. Discoglossus [ 25]; Chalcides [ 47, 20]), for which human introduction elsewhere at circum-Mediterranean sites has been demonstrated and for which African-Sicilian relationships have never been demonstrated using any nuclear sequence data.
Under the most parsimonious scenario, the Plantae share a unique common branch that defines the point of entry of the primary endosymbiont [1], [5], [6], [7], although the monophyly of this group remains to be unambiguously demonstrated using phylogenetic analysis of nuclear genes [8], [9].
Expression of p53 protein has been demonstrated using immunohistochemistry in high-nuclear-grade DCIS (comedo type) [ 25].
We previously demonstrated using immunofluorescence that total Smad2/3 nuclear staining was significantly greater in IPF-derived HLMFs in comparison to NFC-derived cells [ 30], a finding also confirmed in this study (P = 0.0365).
The developed design methodology was demonstrated using FAs of a reference cycle of the BWR6 nuclear power plant.
In fact, at nuclear level the most likely consequence of vitrification would be the necrosis process, which can be better demonstrated using careful morphological and ultrastructural analysis [ 16].
This strategy was demonstrated by using nuclear factor-kappa B p50 (NF-κB p50) and specific recognition sequences as a model case.
We demonstrate using log likelihood scores.
In search for a parallel pathway for 5mC demethylation, a decarboxylation activity toward 5caC was indeed demonstrated in a cell-free system using nuclear extracts of mESCs.
Interestingly, in vitro peptide pull down assays using nuclear HeLa extracts demonstrated that PRC2 binding to the H3K27me3 peptide is completely abolished when the neighboring S28 residue is phosphorylated, suggesting that the phosphorylation status of H3S28 can affect PcG binding in vivo [64] (Fig. 1A).
