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Lastly, multiplexed bio-detection was demonstrated using human serum albumin (HSA) and human C-reactive protein (CRP) as model target proteins.
In vitro, PVL induction of IL8 could be demonstrated using human neutrophils at a concentration lower than that required to induce cell lysis [37].
Previously we demonstrated, using human primary cell cultures, that co-culturing stroma with epithelial cells grown as 3D spheroids increased lateral epithelial cell adhesion [4], whilst in monolayer stroma causes scattering of epithelial cells [5].
Importantly, we demonstrated using human uninfected CD8+-depleted PBMCs that prostratin did not increase the cytotoxicity of these HDACIs, except for the MS-275 which exhibited a slight increased cellular toxicity when combined with prostratin (Figure S2, compare panel A and B; Text S2).
The applicability of the elaborated method was demonstrated using human urine samples.
An age-dependent reduction in H4K16 acetylation levels was also demonstrated using human oocytes [ 67].
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Objectives: To demonstrate, using human factors engineering (HFE), that a redesigned, pre-filled, ready-to-use, pre-asembled follitropin alfa pen can be used to administer prescribed follitropin alfa doses safely and accurately.
Furthermore, it has also been demonstrated using osteosarcoma human cell line that quiescent cells (as defined by cells grown to confluence for several days) have 5-fold less DNA-PK activity than proliferating cells [49].
A similar effect was demonstrated using spiked human urine samples; thus, in the data that follows, VEGF levels must be interpreted in the light of measured Suramin concentrations and reflect free VEGF concentrations.
We have demonstrated using fixed human cell lines that CDC25B localizes to the centrosome throughout the cell cycle (Schmitt et al., 2006; Boutros et al., 2007b; Boutros and Ducommun, 2008).
In the current study, a fraction of the Japanese population, including both healthy subjects and HD patients, exhibited 10-fold higher levels of serum vaspin of more than 10 ng/ml, as demonstrated using the human vaspin RIA system with wider concentration ranges of measurement.
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