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We demonstrated tight coupling between the modular chassis and ester modules for enhanced product biosynthesis, an engineered phenotype useful for directed metabolic pathway evolution.
We confirmed the expression of the K13-ERTAM protein by immunoblotting (Figure 1A) and demonstrated tight regulation of its activity upon 4OHT treatment by immunofluorescence staining for p65/RelA subunit of NF-κB.
In both the repressor and activator configurations, we have demonstrated tight control of gene expression both in transient and stable expression.
Further studies demonstrated tight association of VSM cells with the microvasculature in control mice and the presence of α-SMA+ cells close to the airway subepithelium in HDM-exposed mice (Fig. 4, E– L).
Our study demonstrated tight control of Cdc6 dephosphorylation that likely relies on a continuous route for Cdc6 entry to the active site formed by PR70 and the catalytic subunit, which is affected by the interaction between two subunits.
The previously demonstrated tight link between longevity and fecundity is weakening since methionine addition, one of the essential amino acids, has been demonstrated to be sufficient to increase fecundity at the same extent of full feeding but had no influence on lifespan, raising the possibility that the trade-off between fecundity and longevity is not a mandatory scenario [ 47].
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UV Vis spectroscopy demonstrates tight 1 1 complex formation between Co II) and IAA.
More importantly, these modifications do not alter the level of transgene expression in non-NA cells either in vitro or in vivo, demonstrating tight cell-type specificity.
A fluidic MEMS module – a prototype gas reformer – was bonded onto the carrier to demonstrate tight gas connections at elevated temperature.
Xylitol production by the wild-type strain (W3110) expressing CbXR is not reduced when xylE and xylG are deleted, demonstrating tight regulation of the xylose transporters by CRP and revealing significant secondary xylose transport.
ECCs demonstrate tight crack width and development of these cracks in fact increase ability of ECCs to resist effects of hot, frost and humid weather conditions besides its low permeability coefficient and higher resistance to steel corrosion compared to other common substitutes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com