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However, the fact that 12 of the 13 (92%) CpGs representing potential methylation-mediated relationships between genotype and EOC risk demonstrated the same direction of association with EOC status is encouraging and serves as motivation for the continued and future study of these markers.
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For the genes where all tested individuals of a population demonstrated AEI in the same direction (see Figure 1), it is difficult to detect any regulatory variants using the LR model.
Results were considered consistent (Table 3) when at least 75%% of the studies demonstrated results in the same direction, according to statistical significance of p < 0.05.
(d) CMR with phase-contrast analysis demonstrates the same caudal direction of flow of the anomalous vein and the descending aorta.
Reassuringly, another control gene GUSB (which was not used for normalisation of VHL/CA9 in this study) demonstrates variation in the same direction with reduced expression in the normal tissues (average CT=23.3) compared with tumour tissues (average CT=22.2).
For each of these factors, the evidence from randomised controlled trials, from meta-analyses of randomised controlled trials, and from observational studies demonstrates effects in the same direction and of similar magnitude; details are not provided here but are available from the original review [ 12].
Other putative Wnt target genes from the Bmp pathway (Bmp2, Bmp4, and Grem1) showed significant expression changes in our microarray, with Q-PCR analysis demonstrating changes in the same direction to the arrays in all cases although these were not formally significant (see figure 1B in online supplement).
Of the 58 genes examined by both of these groups, the expression of 17 (29%) changed in the same direction, demonstrating a reasonable agreement between the two studies considering their different designs.
Low heterogeneity is demonstrated by both OR's showing the same direction of treatment effect, moderate is indicated by a neutral effect in one component, and large is seen where the OR's have opposite patterns of risk.
This assay captured an additional CpG site located 38 bp away from cg14752227, which also demonstrated a non-significant trend in the same direction as that seen for cg14752227 (Fig. 6B, right graph).
Perner agrees that the evidence points in the same direction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com