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Accordingly, Fabriek and coworkers demonstrated the binding of Gram-positive and Gram-negative bacteria to CD163 and induction of inflammatory cytokines in CD163-expressing CHO cells and suppression of bacteria-induced cytokines in human monocytes by blocking antibodies against CD163 [19].
By ectopic expression, Gaudio et al demonstrated the binding of c-Jun with human WWOX and shown inhibition of c-Jun-regulated promoter activation by WWOX [42].
Several studies demonstrated the binding of HIV-1 to RBCs through DARC enabling RBCs to transmit HIV to peripheral blood mononuclear cells.
In-vitro analyses have previously demonstrated the binding of c-MYC to cis-acting canonical E-boxes CACGTG and CATGTG [36] as well as non-canonical variants.
Electrophoretic mobility shift assays (EMSA) and supershift analysis demonstrated the binding of transcription factor Elk-1 within this promoter region.
To this end, we performed FCS studies and demonstrated the binding of A38 to VACV particles, but not to CMV or latex beads of similar size.
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The results demonstrate the binding of anti-ricin A chain MAb (RAC18) to rPAC and rRAC, but not the unrelated isotype control.
UV-vis spectra showed anapproximately 450 nm absorption peak (Figure 6A), demonstrating the binding of FITC to nrGO-PEG.
To demonstrate the binding of HIF-1α protein to the promoter of CX3CR1, the ChIP assay was performed with a commercial kit (Upstate Biotechnology; Lake Placid, NY; www.upstatebiotech.com) using the manufacturer's protocol with minor adjustments.
To demonstrate the binding of AR to this promoter region, we carried out Chromatin-IP analysis.
Electromobility shift assays then showed specific signals demonstrating the binding of protein complexes to LTSM in ribosomal protein gene promoters.
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