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For the statistical analysis, two samples that had demonstrated quantities of methylation between 10 to 25% on MS-HRM but had insufficient DNA for pyrosequencing were also included.
A higher cut-off was chosen for pyrosequencing to ensure that only significant quantities of methylation were included on the basis that unmethylated DNA controls (WGA) demonstrated quantities of methylation up to 8% at individual CpG sites (see Additional file 2: Table S4).
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Postoperatively, the patient demonstrated significant quantities of milky fluid drainage from the right pleural chest tube.
Immunocytochemical studies demonstrated increased quantities of immunoreactive aromatase in both cell types as a result of these treatments.
This demonstrated the quantity of this assay (γ=0.999).
We also established the ligand-binding abilities of mt-nAChRs and demonstrated that quantity of the mt-nAChRs coupled to inhibition of mPTP opening increases upon malignant transformation.
We also established the ligand-binding abilities of mt-nAChRs and demonstrated that quantity of the mt-nAChRs coupled to inhibition of mPTP opening increases upon malignant transformation of lung cells.
Increased red cell destruction is recognized by demonstrating increased quantities of the pigmentary products of their destruction, such as bilirubin and urobilinogen, in the blood plasma, urine, and feces and by evidence of accelerated erythropoiesis, such as an increase in the number of young cells (reticulocytes) in the blood.
On pyrosequencing, only four samples (4/78) demonstrated mean methylation quantities greater than 15%.
In tumors that express low levels of PgP, it has been demonstrated that large quantities of drug remain in the cell layers closest to blood vessels, while distal cells acquire little to no drug [ 7, 22].
Thus, it was demonstrated that the quantities of VLY required to induce the lysis of HeLa cells are higher than that required to induce the lysis of human erythrocytes (2.5 μg/ml and 5 ng/ml, respectively).
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