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For ER and PR, a breast tumour was regarded positive when at least 10% of the tumour cell nuclei demonstrated protein expression.
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Western blot demonstrating protein expression in HEK293 cell lysates is shown for all constructs in Figure S7A.
This experimental study is the first to demonstrate protein expression of GLUT2 and SVCT2 in the human bronchial epithelium.
A representative experiment demonstrating protein expression of Notch-1 in HaCaT keratinocytes 24 h following treatment with UV-B [(A) 0 J; (B) 100 J; (C) 500 J] alone or together with 1,25(OH)2D3 (10−8 M) is shown in Figure 2.
A representative experiment demonstrating protein expression of Jagged-1 in HaCaT keratinocytes 24 h following treatment with UV-B (A: 0 J; B: 100 J; (C) 500 J] alone or together with 1,25(OH)2D3 (10−8 M) is shown in Figure 3.
As shown in Figure 3, the staining demonstrated NOS3 protein expression predominantly within the airway epithelium and again subjects with AA genotype demonstrated higher NOS3 expression compared with AG and GG individuals.
All of the other genomic elements demonstrated some protein expression and the largest linear plasmid had 53 expressed proteins or 9% of the total ORFs on this plasmid.
Similar to western blotting analysis, immunocytochemistry also demonstrated dystrophin protein expression in Control-Myocytes but not in DMD-Myocytes (Fig. 3d).
Immunohistochemical staining demonstrated TrkB protein expression in tumor epithelial cells at the outer edges of tumors, as well as adjacent stroma and glandular epithelium.
Overall, our studies demonstrated that protein expression profiles of adhesion molecules, cytokeratin, and proteases could predict the strategy of penetration/invasion using an in vitro 3D model, although, there were some exceptions.
Moreover, Western blot using specific antibodies against activated RELA and STAT5B demonstrated higher protein expression levels in SKOV3-CR cells and to a lesser extent in A2780-CR cells prior to knockdown as compared to their naïve counterparts (Figure 2E).
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