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Intravenous administration of the optimized polyplexes in mice led to gene expression in the brain, which upon further immunohistochemical analysis demonstrated gene expression in neurons.
We further demonstrated gene expression of HNF6-regulated genes, namely, HSP105B, CYP51, and C/EBPα to be significantly upregulated upon siRNA-mediated functional knockdown of FOXA2.
Notably, all three PBMC samples collected one year after baseline demonstrated gene expression nearly identical to the initial samples (yellow bars, Figure 1A).
Later, these patients demonstrated gene expression changes consistent with simultaneous, persistent pro-inflammatory and immunosuppressive states.
The comparison demonstrated gene expression as H. glycines experienced an R or S reaction.
Other analyses demonstrated gene expression in H. glycines experiencing R or S reactions during the course of infection.
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Our results demonstrate gene expression differences between the majority of lSSc patients and healthy controls, as well as differences between lSSc-PAH and lSSc-NoPAH patients.
We also demonstrate gene expression changes associated with thyroid histopathology and hormone status after PB and PTU exposures.
As transcriptomics only demonstrates gene expression, not translation or activity, these hypotheses cannot be confirmed with this data alone.
For both cultivars, total RBCS1 expression values were similar before (U1) and after (U2) the WS period, demonstrating gene expression recovery with the return of irrigation.
Numerous differential expression analyses are presented demonstrating gene expression in H. glycines experiencing R or S reactions at single time points.
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