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In addition, the analysis of 30 samples, derived from children with T-ALL, demonstrated expressions of Notch3 and its target gene HES-1, as well as of pT α transcripts.
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Northern blot analysis demonstrated expression of the variant mRNAs.
Transcriptome analyses showed that 11,328 of the oligonucleotides demonstrated expression in at least one tissue.
Full-length cloning demonstrated expression of the long variant in endothelial cells.
We discovered neutralizing antibodies to IL-1RA in a subset of IgG4-RD patients and demonstrated expression of IL-1RA within inflamed IgG4-RD lesions of multiple organs.
Engineered T cells demonstrated expression of ectopically-delivered TCRs, resulting in endowment of cognate antigen-specific responses.
Immunohistochemistry demonstrated expression of synaptophysin, chromogranin A, and CD56.
Immunofluorescence imaging demonstrated expression of HA-SSTR2 on the cell membrane as expected (Fig. 1).
Sequence analysis of randomly selected clones demonstrated expression of both identified (calmodulin, SNARE protein, syndecan-2) and unidentified genes, and confirmed synthesis of full length transcripts.
However, several laboratories have now demonstrated expression of RAG-1 and RAG-2 and active V-to- D J recombination in germinal center B cells.
Lastly, we demonstrated expression of EBNA1, a protein essential for maintaining viral episome.
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