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In starlings, GnIH receptor (GPR147) mRNA is expressed in GnRH neurons [19] and we demonstrated expression of the GPR147 in human hypothalamus.
None of them demonstrated expression of the AQP8 transcript, supporting the hypothesis that tumor cells do not express AQP8 (Fig. 4).
Northern blot analysis demonstrated expression of the variant mRNAs.
Full-length cloning demonstrated expression of the long variant in endothelial cells.
Immunoprecipitation of HA tagged HIF-1α-PPN from heart protein lysates demonstrated expression of the transgene (Figure 2A).
Using a similar approach, we demonstrated expression of the same set of early genes but no post-replication genes even when analyzed 12 h after infection.
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Differentiated RGC-5s also had prominent TrkB staining, demonstrating expression of the high-affinity BDNF receptor.
It was used to demonstrate expression of the pyruvate decarboxylase (pdc) gene from Zymomonas palmae in G. thermoglucosidasius at 45 oC.
RT-PCR analysis was used to demonstrate expression of the human cDNA (Fig. 8B).
The generation of the murine vLINCL cell panel is described in detail in Materials and Methods, along with the characterization demonstrating expression of the neural stem cell marker, nestin, confirming a neuronal lineage (Figure S1).
This concept has been challenged by reports demonstrating expression of the VEGFRs on tumor cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com