Removal of N-terminal amino acids up to the first C2H2 domain (-N99) caused significant reduction of β-galactosidase activity demonstrating essentiality of this domain for transactivation.
Two homologs of RuvB in Saccharomyces cerevisiae (scRuvBL1 and scRuvBL2) have been studied in detail and the knockout of scRuvBL1 demonstrated its essentiality in yeast growth.
Yet, Lemon -- newly emerging from his bold middle passage as the shamanic interpreter between epochs -- demonstrates the essentiality of this marriage of inner and outer.
Curing of this "rescue" plasmid from the bacterial population resulted in a cessation of growth, demonstrating gene essentiality.
Drastic reduction of osmotolerance in the hog1 mutants demonstrates the essentiality of this module in hyperosmotic stress.
Some methanogen enzymes or pathways that have been previously targeted by researchers for inhibition demonstrating the essentiality of certain enzymes/pathways were also taken into consideration.
The addition of the SHMT inhibitor and amino acid analog, aminomethylphosphonate, to the reaction mixture inhibited dTMP synthesis by greater than 95%, demonstrating the essentiality of the SHMT reaction in generating folate-activated one-carbons from serine for dTMP synthesis in nuclei.
To formally demonstrate the essentiality of ssrA and smpB, H. pylori strain N6 was first transformed by stably replicating plasmids pILL788 and pILL786 expressing ssrA or smpB, respectively, under control of an IPTG inducible promoter derived from pILL2150 [30] (Tables 1 and S1).
Future research may focus on the functionality of Dicers without the PAZ domain to demonstrate the essentiality of the domain in fungi.
The anti-HAF antibody completely blocks acid secretion in histamine-stimulated rabbit gastric glands demonstrating the essentiality of the HAF in gastric secretory process.
