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The Foxp3 gene contains several conserved potential STAT5 binding sites, some of which demonstrated binding of STAT5 in Treg but not Tconv (Burchill et al., 2007; Yao et al., 2007).
Further, we have also demonstrated binding of a biotinylated-BH4-BCL2 peptode to cells in vitro (Fig. S4).
Pull-down assay demonstrated binding of nucleolin and Stat1 in M-CSFR expressing THP-1 cells stimulated with CSF-1 (Fig. 3B).
Ligand affinity blot demonstrated binding of CFH to G1/pCRASP-3 and G1/pCRASP-5 but not G1 and G1/pKFSS1 (Fig. 3E and F, respectively).
Having demonstrated binding of CFHRs to intact borrelial cells, the role of CFHR for complement resistance was assayed under more physiological conditions.
In this context, we recently demonstrated binding of R5P to Pdx1 by fluorescence spectroscopy through the fortuitous presence of a single tryptophan residue at the very C terminus of the B. subtilis protein.
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The interface suggests that EphA family members bind to SHIP2 SAM, whereas EphB members may not; correspondingly, we demonstrate binding of EphA1, but not of EphB2, to SHIP2.
EMSA and DPA were used to demonstrate binding of CovR to P1882.
However, they failed to demonstrate binding of a recombinant IZUMO1 peptides with the eggs.
To our knowledge, this is the first report which demonstrates binding of CFHR2 and CFHR5 to a human pathogen.
Our approach demonstrates binding of EWS-FLI1 to GGAA-repeat sequences in vivo and further shows a binding preference for tracts of 9 repeats or more.
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